Gregor Hagelueken's Publications

Our latest publications can always be found on Google Scholar.

2023
de Regt, A.K., Anand, K., Ciupka, K., Bender, F., Gatterdam, K., Putschli, B., Fusshöller, D., Hilbig, D., Kirchhoff, A., Hunkler, C., Wolter, S., Grünewald, A., Wallerath, C., Schuberth-Wagner, C., Ludwig, J., Paeschke, K., Bartok, E., Hagelueken, G., Hartmann, G., Zillinger, T., Geyer, M., and Schlee, M. (2023). A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNA. Nucleic Acids Res., gkad835. doi: 10.1093/nar/gkad835.

Vitali V, Ackermann K, Hagelueken G, Bode BE, "Spectroscopically Orthogonal Labelling to Disentangle Site-Specific Nitroxide Label Distributions." Appl. Magn. Res. (2023) doi:10.1007/s00723-023-01611-1

Wirtz DA, Schneberger N, Klöppel S, Richarz R, Geyer M, König GM, Hagelueken G, Crüsemann M, "Adenylation Domain-Guided Recruitment of Trans-Acting Nonheme Monoxygenases in Nonribosomal Peptide Biosynthesis." ACS Chem. Biol. (2023) doi:10.1021/acschembio.3c00106 

Peter MF, Ruland JA, Kim Y, Hendricks P, Siebrasse JP, Thomas GH, Kubitscheck U, Hagelueken G, "Conformational coupling of the sialic acid TRAP transporter HiSiaQM with its substrate binding protein HiSiaP." bioRxiv (2023) doi:10.1101/2023.03.04.531103

Rouillon C, Schneberger N, Chi H, Blumenstock K, Da Vela S,  Ackermann K, Moecking J, Peter MF, Boenigk W, Seifert R, Bode BE, Schmid-Burgk J-L, Svergun D, Geyer M, White MF and Hagelueken G, "Antiviral signaling by a cyclic nucleotide activated CRISPR protease." Nature (2023) 614:168-174, doi:10.1038/s41586-022-05571-7

2022
Moecking J, Laohamonthonkul P, Meşe K, Hagelueken G, Steiner A, Harapas CR, Sandow JJ, Graves JD, Masters SL and Geyer M, "Inflammasome sensor NLRP1 disease variant M1184V promotes autoproteolysis and DPP9 complex formation by stabilizing the FIIND domain." J. Biol. Chem. (2022) 298:102645, doi:10.1016/j.jbc.2022.102645

Peter MF, Ruland JA, Depping P, Schneberger N, Severi E, Moecking J, Gatterdam K, Tindall S, Durand A, Heinz V, Siebrasse J-P, Koenig P-A, Geyer M, Ziegler C, Kubitscheck U, Thomas GH and Hagelueken G, "Structural and mechanistic analysis of a tripartite ATP-independent periplasmic TRAP transporter", Nat. Commun. (2022) 13:4471 doi:10.1038/s41467-022-31907-y

Peter MF, Gebhardt C, Mächtel R, Moya Muñoz GG, Glaenzer J, Narducci A, Thomas GH, Cordes T and Hagelueken G, "Cross-validation of distance measurements in proteins by PELDOR/DEER and single-molecule FRET", Nat. Commun. (2022) 13:4396, doi:10.1038/s41467-022-31945-6 

Hochheiser IV, Behrmann H, Hagelueken G, Rodríguez-Alcázar JF, Kopp A, Latz E, Behrmann E and Geyer M, "Directionality of PYD filament growth determined by the transition of NLRP3 nucleation seeds to ASC elongation", Sci. Adv. (2022) 8:eabn7583, doi:10.1126/sciadv.abn7583

Hochheiser IV, Pilsl M, Hagelueken G, Moecking J, Marleaux M, Brinkschulte R, Latz E, Engel C and Geyer M, "Structure of the NLRP3 decamer bound to the cytokine release inhibitor CRID3", Nature (2022) 604:184-189, doi:10.1038/s41586-022-04467-w

2021
Rouillon C, Schneberger N, Chi H, Peter MF, Geyer M, Boenigk W, Seifert R, White MF and Hagelueken G, "SAVED by a toxin: Structure and function of the CRISPR Lon protease", bioRxiv (2021) doi:10.1101/2021.12.06.471393

Peter MF, Depping P, Schneberger N, Severi E, Gatterdam G, Tindall S, Durand A, Heinz V, Koenig P-A, Geyer M, Ziegler C, Thomas GH and Hagelueken G, "The structure of HiSiaQM defines the architecture of tripartite ATP-independent periplasmic (TRAP) transporters", bioRxiv (2021) doi:10.1101/2021.12.03.471092

Hochheiser IV, Behrmann H, Hagelueken G, Rodríguez-Alcázar JF, Kopp A, Latz E, Behrmann E and Geyer M, "Directionality of PYD filament growth determined by the transition of NLRP3 nucleation seeds to ASC elongation", bioRxiv (2021) doi:10.1101/2021.11.25.470035

Hochheiser IV, Pilsl M, Hagelueken G, Moecking J, Marleaux M, Brinkschulte R, Latz E, Engel C and Geyer M, "Cryo-EM structure of the NLRP3 decamer bound to the cytokine release inhibitory drug CRID3", bioRxiv (2021) doi:10.1101/2021.07.22.453353

Peter MF, Gebhardt C, Glaenzer J, Schneberger N, de Boer M, Thomas GH, Cordes T and Hagelueken G, "Triggering closure of a sialic acid TRAP transporter substrate binding protein through binding of natural and artificial substrates", J. Mol. Biol. (2021) 433:166756, doi:10.1016/j.jmb.2020.166756

2020
Peter MF, Gebhardt C, Mächtel R, Glaenzer J, Thomas GH, Cordes T and Hagelueken G, "Cross-validation of distance measurements in proteins by PELDOR/DEER and single-molecule FRET", bioRxiv (2020) doi:10.1101/2020.11.23.394080

Dominick C, Eggert F, Wuebben C, Bornewasser L, Hagelueken G, Schiemann O and Kath-Schorr S, "EPR Distance Measurements on Long Non-coding RNAs Empowered by Genetic Alphabet Expansion Transcription", Angew. Chem. Int. Ed. Engl. (2020) 59:7891-7896, doi:10.1002/anie.201916447

2019
Jassoy JJ, Heubach CA, Hett T, Bernhard F, Haege FR, Hagelueken G and Schiemann O, "Site Selective and Efficient Spin Labelling of Proteins with a Maleimide-Functionalized Trityl Radical for Pulsed Dipolar EPR Spectroscopy", Molecules (2019) 24:2735, doi:10.3390/molecules24152735


Peter MF, Tuukkanen AT, Heubach CA, Selsam A, Duthie FG, Svergun DI, Schiemann O and Hagelueken G, "Studying Conformational Changes of the Yersinia Type-III-Secretion Effector YopO in Solution by Integrative Structural Biology", Structure (2019) 27:1416-1426, doi:10.1016/j.str.2019.06.007

Abdullin D, Matsuoka H, Yulikov M, Fleck N, Klein C, Spicher S, Hagelueken G, Grimme S, Lützen A and Schiemann O, "Pulsed EPR Dipolar Spectroscopy under the Breakdown of the High-Field Approximation: The High-Spin Iron(III) Case", Chem. Europ. J. (2019) 25:8820-8828, doi:10.1002/chem.201900977

Dominick C, Hagelueken G, Eggert F, Schiemann O and Kath-Schorr S, "Posttranscriptional spin labelling of RNA by tetrazine-based cycloaddition", Org. Biomol. Chem. (2019) 17:1805-1808, doi:10.1039/C8OB02597E

2018
Glaenzer J, Peter MF and Hagelueken G, "Studying structure and function of membrane proteins with PELDOR/DEER spectroscopy - A crystallographers' perspective", Methods (2018) 147:163-175, doi:10.1016/j.ymeth.2018.03.002

Yin DM, Hammler D, Peter MF, Marx A, Schmitz A and Hagelueken G, "Inhibitor-directed spin labelling - a high precision and minimally invasive technique to study the conformation of proteins in solution", Chem. Eur. J. (2018) 24:6665-6671, doi:10.1002/chem.201706047

2017
Bountra K, Hagelueken G, Choudhury HG, Corrade C, El Omari K, Wagner A, Mathavan I, Zirah S, Wahlgren WY, Tieleman DP, Schiemann O, Rebuffat S and Beis K, "Structural basis for antibacterial peptide self-immunity by the bacterial ABC transporter McjD", EMBO J. (2017) 36:3062-3079, doi:10.15252/embj.201797278

Yin DM, Hannam JS, Schmitz A, Schiemann O, Hagelueken G, and Famulok M, "Studying the conformation of a receptor tyrosine kinase in solution by inhibitor based spin labelling", Angew. Chemie Int. Ed. (2017) 56:8417-8421 doi:10.1002/anie.201703154

Glaenzer J, Peter MF, Thomas GH and Hagelueken G, "PELDOR spectroscopy reveals two defined states of a sialic acid TRAP transporter SBP in solution", Biophys. J. (2017) 112:109-120, doi:10.1016/j.bpj.2016.12.010

Brewitz HH, Hagelueken G and Imhof D, "Structural and functional diversity of transient heme binding to bacterial proteins", Biochim. Biophys. Act. (2017) 1861:683-697, doi:10.1016/j.bbagen.2016.12.021

Jassoy JJ, Berndhäuser A, Duthie F, Kühn SP, Hagelueken G and Schiemann O, "Versatile trityl spin labels for nanometer distance measurements on biomolecules in vitro and within cells", Angew. Chemie Int. Ed. (2017) 56:177-181, doi:10.1002/anie.201609085

2016
Kerzhner M, Abdullin D, Więcek J, Matsuoka H, Hagelueken G, Schiemann O and Famulok M, "Post-synthetic spin-labeling of RNA through click chemistry for PELDOR measurements", Chem. Eur. J. (2016) 22:12113–12121, doi:10.1002/chem.201601897

Hagelueken G, Hoffmann J, Schubert E, Duthie FG, Florin N, Konrad L, Imhof D, Behrmann E, Morgner N and Schiemann O, "Studies on the X-ray and solution structure of FeoB from Escherichia coli BL21", Biophys. J. (2016) 110:2642–2650, doi:10.1016/j.bpj.2016.05.018

Brewitz HH, Goradia N, Schubert E, Galler K, Kühl T, Syllwasschy B, Popp J, Neugebauer U, Hagelueken G, Schiemann O, Ohlenschläger O, Imhof D, "Heme interacts with histidine- and tyrosine-based protein motifs and inhibits enzymatic activity of chloramphenicol acetyltransferase from Escherichia coli", Biochim. Biophys. Acta (2016) 1860:1343–1353, doi:10.1016/j.bbagen.2016.03.027

Abdullin D, Hagelueken G and Schiemann O, "Determination of nitroxide spin label conformations via PELDOR and X-ray crystallography", Phys. Chem. Chem. Phys. (2016) 18:10428–10437, doi:10.1039/c6cp01307d

2015
Abdullin D, Duthie FG, Meyer A, Müller ES, Hagelueken G and Schiemann O, "Comparison of PELDOR and RIDME for distance measurements between nitroxides and low-spin Fe(III) ions", J. Phys. Chem. B. (2015) 119:13534–13542, doi:10.1021/acs.jpcb.5b02118

Hagelueken G, Duthie FG, Florin N, Schubert E and Schiemann O, "Expression, purification and spin labelling of the ferrous iron transporter FeoB from Escherichia coli BL21 for EPR studies", Protein Expr. Purif. (2015) 114:30–36, doi:10.1016/j.pep.2015.05.014

Schubert E, Florin N, Duthie FG, Brewitz HH, Kühl T, Imhof D, Hagelueken G and Schiemann O, "Spectroscopic studies on peptides and proteins with cysteine-containing heme regulatory motifs (HRM)", J. Inorg. Biochem. (2015) 148:49–56, doi:10.1016/j.jinorgbio.2015.05.008

Abdullin D, Florin N, Hagelueken G and Schiemann O, "EPR-based approach for the localization of paramagnetic metal ions in biomolecules", Angew. Chem. Int. Ed. (2015) 54:1827–1831, doi:10.1002/anie.201410396

Hagelueken G, Clarke BR, Huang H, Tuukkanen A, Danciu I, Svergun DI, Hussain R, Liu H, Whitfield C and Naismith JH, "A coiled-coil domain acts as a molecular ruler to regulate O-antigen chain length in lipopolysaccharide", Nat. Struct. Mol. Biol (2015) 22:50–56, doi:10.1038/nsmb.2935

Hagelueken G, Abdullin D and Schiemann O, "mtsslSuite: Probing biomolecular conformation by spin-labeling studies", Meth. Enzymol. (2015) 563:595–622, doi:10.1016/bs.mie.2015.06.006

2014
Abdullin D, Hagelueken G, Hunter RI, Smith GM and Schiemann O, "Geometric model-based fitting algorithm for orientation-selective PELDOR data", Mol. Phys. (2014) 113:544–560, doi:10.1080/00268976.2014.960494

Ward R, Pliotas C, Branigan E, Hacker C, Rasmussen A, Hagelueken G, Booth IR, Miller S, Lucocq J, Naismith JH and Schiemann O, "Probing the structure of the mechanosensitive channel of small conductance in lipid bilayers with pulsed electron-electron double resonance", Biophys. J. (2014) 106:834–842, doi:10.1016/j.bpj.2014.01.008

Florin N, Schiemann O and Hagelueken G, "High-resolution crystal structure of spin labelled (T21R1) azurin from Pseudomonas aeruginosa: a challenging structural benchmark for in silico spin labelling algorithms", BMC Struct. Biol. (2014) 14:16, doi:10.1186/1472-6807-14-16


2013
Matalon E, Huber T, Hagelueken G, Graham B, Frydman V, Feintuch A, Otting G and Goldfarb D, "Gadolinium(III) spin labels for high-sensitivity distance measurements in transmembrane helices", Angew. Chem. Int. Edit. (2013) 52:11831-11834, doi:10.1002/anie.201305574

Branigan E, Pliotas C, Hagelueken G and Naismith JH, "Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding", Nat. Protoc. (2013) 8:2090–2097, doi:10.1038/nprot.2013.128

Hagelueken G, Abdullin D, Ward R and Schiemann O, "mtsslSuite: In silico spin labelling, trilateration and distance-constrained rigid body docking in PyMOL", Mol. Phys. (2013) 111:2757–2766, doi:10.1080/00268976.2013.809804

2012
Hagelueken G, Huang H, Clarke BR, Lebl T, Whitfield C and Naismith JH, "Structure of WbdD: a bifunctional kinase and methyltransferase that regulates the chain length of the O antigen in Escherichia coli O9a", Mol. Microbiol. (2012) 86:730–742, doi:10.1111/mmi.12014

Pliotas C, Ward R, Branigan E, Rasmussen A, Hagelueken G, Huang H, Black SS, Booth IR, Schiemann O and Naismith JH, "Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy", Proc. Nat. Acad. Sci. U.S.A. (2012) 109:E2675–82, doi:10.1073/pnas.1202286109

Hagelueken G, Huang H, Harlos K, Clarke BR, Whitfield C and Naismith JH, "Crystallization, dehydration and experimental phasing of WbdD, a bifunctional kinase and methyltransferase from Escherichia coli O9a", Acta Cryst. D Biol. Crystallogr. (2012) 68:1371–1379, doi:10.1107/S0907444912029599

Hagelueken G, Ward R, Naismith JH and Schiemann O, "MtsslWizard: In silico spin-labeling and generation of distance distributions in PyMOL", Appl. Magn. Reson. (2012) 42:377–391, doi:10.1007/s00723-012-0314-0

2010
Steiner K, Hagelueken G, Messner P, Schäffer C and Naismith JH, "Structural basis of substrate binding in WsaF, a rhamnosyltransferase from Geobacillus stearothermophilus", J. Mol. Biol. (2010) 397:436–447, doi:10.1016/j.jmb.2010.01.035

2009
Hagelueken G, Huang H, Mainprize IL, Whitfield C and Naismith JH, "Crystal structures of Wzb of Escherichia coli and CpsB of Streptococcus pneumoniae, representatives of two families of tyrosine phosphatases that regulate capsule assembly", J. Mol. Biol. (2009) 392:678–688, doi:10.1016/j.jmb.2009.07.026

Huang H, Hagelueken G, Whitfield C and Naismith JH, "Crystallization and preliminary crystallographic analysis of the bacterial capsule assembly-regulating tyrosine phosphatases Wzb of Escherichia coli and Cps4B of Streptococcus pneumoniae", Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. (2009) 65:770–772, doi:10.1107/S1744309109023914

Hagelueken G, Ingledew WJ, Huang H, Petrovic-Stojanovska B, Whitfield C, ElMkami H, Schiemann O and Naismith JH, "PELDOR spectroscopy distance fingerprinting of the octameric outer‐membrane protein Wza from Escherichia coli", Angew. Chem. Int. Edit. (2009) 48:2904–2906, doi:10.1002/anie.200805758

Hagelueken G, Albrecht SC, Steinmetz H, Jansen R, Heinz DW, Kalesse M and Schubert W-D, "The absolute configuration of rhizopodin and its inhibition of actin polymerization by dimerization", Angew. Chem. Int. Edit. (2009) 48:595–598. doi:10.1002/anie.200802915

2007
Hagelueken G, Wiehlmann L, Adams TM, Kolmar H, Heinz DW, Tümmler B and Schubert W-D, "Crystal structure of the electron transfer complex rubredoxin rubredoxin reductase of Pseudomonas aeruginosa", Proc. Nat. Acad. Sci. U.S.A. (2007) 104:12276–12281, doi:10.1073/pnas.0702919104

2006
Hagelueken G, Adams TM, Wiehlmann L, Widow U, Kolmar H, Tümmler B, Heinz DW and Schubert W-D, "The crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, defines a third class of sulfatases", Proc. Nat. Acad. Sci. U.S.A. (2006) 103:7631–7636, doi:10.1073/pnas.0510501103

2005
Eiting M, Hagelüken G, Schubert WD and Heinz DW, "The mutation G145S in PrfA, a key virulence regulator of Listeria monocytogenes, increases DNA-binding affinity by stabilizing the HTH motif", Mol. Microbiol. (2005), 56:433–446, doi:10.1111/j.1365-2958.2005.04561.x