Publications of the ISB Bonn

(since Nov. 2017)

2022
Hochheiser, I.V. and Geyer, M. (2022). An assay for the seeding of homotypic Pyrin domain filament transitions. Methods Mol. Biol. 2523, 197–207.

Moecking, J. and Geyer, M. (2022). A surface plasmon resonance-based strategy to characterize interactions of NLR proteins with associated factors. Methods Mol. Biol. 2523, 161–177.

Hochheiser, I.V., Behrmann, H., Hagelueken, G., Rodríguez-Alcázar, J.F., Kopp, A., Latz, E., Behrmann, E., and Geyer, M. (2022). Directionality of PYD filament growth determined by the transition of NLRP3 nucleation seeds to ASC elongation. Sci. Adv. 8, eabn7583.

Steiner, A., Hrovat-Schaale, K., Prigione, I., Yu, C.H., Laohamonthonkul, P., Harapas, C.R., Low, R.R.J., De Nardo, D., Dagley, L.F., Mlodzianoski, M.J., Rogers, K.L., Zillinger, T., Hartmann, G., Gantier, M.P., Gattorno, M., Geyer, M., Volpi, S., Davidson, S., and Masters, S.L. (2022). Deficiency in coatomer complex I causes aberrant activation of STING signalling. Nat. Commun. 13:2321.

Hassoun, R., Erdmann, C., Schmitt, S., Fujita-Becker, S., Mügge, A., Schröder, R.R., Geyer, M., Borbor, M., Jaquet, K., Hamdani, N., and Mannherz, H.G. (2022). Functional characterization of cardiac actin mutants causing hypertrophic (p.A295S) and dilated cardiomyopathy (p.R312H and p.E361G). Int. J. Mol. Sci. 23:4465.

Hochheiser, I.V., Pilsl, M., Hagelueken, G., Moecking, J., Marleaux, M., Brinkschulte, R., Latz, E., Engel, C., and Geyer, M. (2022). Structure of the NLRP3 decamer bound to the cytokine release inhibitor CRID3. Nature 604, 184–189.

Funke, K., Düster, R., De-Graft Wilson, P., Arévalo, L., Geyer, M., and Schorle, H. (2022). Transcriptional CDK inhibitors as potential treatment option for testicular germ cell tumors. Cancers 14:1690.
Harapas, C.R., Idiiatullina, E., Al-Azab, M., Hrovat-Schaale, K., Reygaerts, T., Steiner, A., Laohamonthonkul, P., Davidson, S., Yu, C.H., Booty, L., and Masters, S.L. (2022). Organellar homeostasis and innate immune sensing. Nat. Rev. Immunol. Feb 23. doi: 10.1038/s41577-022-00682-8.

Düster, R., Ji, Y., Pan, K.T., Urlaub, H., and Geyer, M. (2022). Functional characterization of the human Cdk10/Cyclin Q complex. Open Biol. 12:210381.

Keuler, T., Ferber, D., Marleaux, M., Geyer, M., and Gütschow, M. (2022). Structure-stability relationship of NLRP3 inflammasome-inhibiting sulfonylureas. ACS Omega 7, 8158–8162.

Steiner, A., Reygaerts, T., Pontillo, A., Ceccherini, I., Moecking, J., Moghaddas, F., Davidson, S., Caroli, F., Grossi, A., Castro, F.F.M., Kalil, J., Gohr, F.N., Schmidt, F.I., Bartok, E., Zillinger, T., Hartmann, G., Geyer, M., Gattorno, M., Mendonça, L.O., and Masters, S.L. (2022). Recessive NLRC4-autoinflammatory disease reveals an Ulcerative Colitis locus. J. Clin. Immunol. 42, 325–335.


2021
Ludwig, K.U., Schmithausen, R.M., Li, D., Jacobs, M.L., Hollstein, R., Blumenstock, K., Liebing, J., Słabicki, M., Ben-Shmuel, A., Israeli, O., Weiss, S., Ebert, T.S., Paran, N., Rüdiger, W., Wilbring, G., Feldman, D., Lippke, B., Ishorst, N., Hochfeld, L.M., Beins, E.C., Kaltheuner, I.H., Schmitz, M., Wöhler, A., Döhla, M., Sib, E., Jentzsch, M., Borrajo, J.D., Strecker, J., Reinhardt, J., Cleary, B., Geyer, M., Hölzel, M., Macrae, R., Nöthen, M.M., Hoffmann, P., Exner, M., Regev, A., Zhang, F., and Schmid-Burgk, J.L. (2021). LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding. Nat. Biotechnol. 39, 1556–1562.

Kaltheuner, I.H., Anand, K., Moecking, J., Düster, R., Wang, J., Gray, N.S., and Geyer, M. (2021). Abemaciclib is a potent inhibitor of DYRK1A and HIP kinases involved in transcriptional regulation. Nat. Commun. 12:6607.

Cesarato, N., Wehner, M., Ghughunishvili, M., Schmidt, A., Axt, D., Thiele, H., Lentze, M.J., Has, C., Geyer, M., Basmanav, F.B., and Betz, R.C (2021). Four hypotrichosis families with mutations in the gene LSS presenting with and without neurodevelopmental phenotypes. Am. J. Med. Genet. A 185, 3900–3904.

Jiang, B., Jiang, J., Kaltheuner, I.H., Iniguez, A.B., Anand, K., Ferguson, F.M., Ficarro, S.B., Seong, B.K.A., Greifenberg, A.K., Dust, S., Kwiatkowski, N.P., Marto, J.A., Stegmaier, K., Zhang, T., Geyer, M., and Gray, N.S. (2021). Structure-activity relationship study of THZ531 derivatives enables the discovery of BSJ-01-175 as a dual CDK12/13 covalent inhibitor with efficacy in Ewing sarcoma. Eur. J. Med. Chem. 221:113481.

Walch, P., Selkrig, J., Knodler, L.A., Rettel, M., Stein, F., Fernandez, K., Viéitez, C., Potel, C.M., Scholzen, K., Geyer, M., Rottner, K., Steele-Mortimer, O., Savitski, M.M., Holden, D.W., and Typas, A. (2021). Global mapping of Salmonella enterica-host protein-protein interactions during infection. Cell Host Microbe 29, 1316–1332.

Erdmann, C., Hassoun, R., Schmitt, S., Kikuti, C., Houdusse, A., Mazur, A.J., Mügge, A., Hamdani, N., Geyer, M., Jaquet, K., and Mannherz, H.G. (2021). Integration of cardiac actin mutants causing hypertrophic (p.A295S) and dilated cardiomyopathy (p.R312H and p.E361G) into cellular structures. Antioxidants 10:1082.

Jiang, B., Gao, Y., Che, J., Lu, W., Kaltheuner, I.H., Dries, R., Kalocsay, M., Berberich, M.J., Jiang, J., You, I., Kwiatkowski, N.P., Riching, K.M., Daniels, D.L., Sorger, P.K., Geyer, M., Zhang, T., and Gray, N.S. (2021). Discovery and resistance mechanism of a selective CDK12 degrader. Nat. Chem. Biol. 17, 675–683.

Moecking, J., Laohamonthonkul, P., Chalker, K., White, M.J., Harapas, C.R., Yu, C.H., Davidson, S., Hrovat-Schaale, K., Hu, D., Eng, C., Huntsman, S., Calleja, D.J., Horvat, J.C., Hansbro, P.M., O'Donoghue, R.J.J., Ting, J.P., Burchard, E.G., Geyer, M., Gerlic, M., and Masters, S.L. (2021). NLRP1 variant M1184V decreases inflammasome activation in the context of DPP9 inhibition and asthma severity. J. Allergy Clin. Immunol. 147, 2134–2145.

Keuler, T., Gatterdam, K., Akbal, A., Lovotti, M., Marleaux, M., Geyer, M., Latz, E., and Gütschow, M. (2021). Development of fluorescent and biotin probes targeting NLRP3. Front. Chem. 9:642273.

Koenig, P.A., Das, H., Liu, H., Kümmerer, B.M., Gohr, F.N., Jenster, L.M., Schiffelers, L.D.J., Tesfamariam, Y.M., Uchima, M., Wuerth, J.D., Gatterdam, K., Ruetalo, N., Christensen, M.H., Fandrey, C.I., Normann, S., Tödtmann, J.M.P., Pritzl, S., Hanke, L., Boos, J., Yuan, M., Zhu, X., Schmid-Burgk, J.L., Kato, H., Schindler, M., Wilson, I.A., Geyer, M., Ludwig, K.U., Hällberg, B.M., Wu, N.C., and Schmidt, F.I. (2021). Structure-guided multivalent nanobodies block SARS-CoV-2 infection and suppress mutational escape. Science 371:eabe6230.

Düster, R., Kaltheuner, I.H., Schmitz, M., and Geyer, M. (2021). 1,6-Hexanediol, commonly used to dissolve liquid-liquid phase separated condensates, directly impairs kinase and phosphatase activities. J. Biol. Chem. 296:100260.

Peter, M.F., Gebhardt, C., Glaenzer, J., Schneberger, N., de Boer, M., Thomas, G.H., Cordes, T., and Hagelueken, G. (2021). Triggering closure of a sialic acid TRAP transporter substrate binding protein through binding of natural or artificial substrates. J. Mol. Biol. 433:166756.

2020
Yu, C.H., Davidson, S., Harapas, C.R., Hilton, J.B., Mlodzianoski, M.J., Laohamonthonkul, P., Louis, C., Low, R.R.J., Moecking, J., De Nardo, D., Balka, K.R., Calleja, D.J., Moghaddas, F., Ni, E., McLean, C.A., Samson, A.L., Tyebji, S., Tonkin, C.J., Bye, C.R., Turner, B.J., Pepin, G., Gantier, M.P., Rogers, K.L., McArthur, K., Crouch, P.J., and Masters, S.L. (2020). TDP-43 triggers mitochondrial DNA release via mPTP to activate cGAS/STING in ALS. Cell 183, 636–649.

Rimel, J.K., Poss, Z.C., Erickson, B., Maas, Z.L., Ebmeier, C.C., Johnson, J.L., Decker, T.M., Yaron, T.M., Bradley, M.J., Hamman, K.B., Hu, S., Malojcic, G., Marineau, J.J., White, P.W., Breault, M., Tao, L., DeRoy, P., Clavette, C., Nayak, S., Damon, L.J., Kaltheuner, I.H., Bunch, H., Cantley, L.C., Geyer, M., Iwasa, J., Dowell, R.D., Bentley, D.L., Old, W.M., and Taatjes, D.J. (2020). Selective inhibition of CDK7 reveals high-confidence targets and new models for TFIIH function in transcription. Genes Dev. 34, 1452–1473.

Mayor-Ruiz, C., Bauer, S., Brand, M., Kozicka, Z., Siklos, M., Imrichova, H., Kaltheuner, I.H., Hahn, E., Seiler, K., Koren, A., Petzold, G., Fellner, M., Bock, C., Müller, A.C., Zuber, J., Geyer, M., Thomä, N.H., Kubicek, S., and Winter, G.E. (2020). Rational discovery of molecular glue degraders via scalable chemical profiling. Nat. Chem. Biol. 16, 1199–1207.

Braun, M., Aguilera, A.R., Sundarrajan, A., Corvino, D., Stannard, K., Krumeich, S., Das, I., Lima, L.G., Meza Guzman, L.G., Li, K., Li, R., Salim, N., Jorge, M.V., Ham, S., Kelly, G., Vari, F., Lepletier, A., Raghavendra, A., Pearson, S., Madore, J., Jacquelin, S., Effern, M., Quine, B., Koufariotis, L.T., Casey, M., Nakamura, K., Seo, E.Y., Hölzel, M., Geyer, M., Kristiansen, G., Taheri, T., Ahern, E., Hughes, B.G.M., Wilmott, J.S., Long, G.V., Scolyer, R.A., Batstone, M.D., Landsberg, J., Dietrich, D., Pop, O.T., Flatz, L., Dougall, W.C., Veillette, A., Nicholson, S.E., Möller, A., Johnston, R.J., Martinet, L., Smyth, M.J., and Bald, T. (2020). CD155 on Tumor Cells Drives Resistance to Immunotherapy by Inducing the Degradation of the Activating Receptor CD226 in CD8+ T Cells. Immunity 53, 805–823.

Marleaux, M., Anand, K., Latz, E., and Geyer, M. (2020). Crystal structure of the human NLRP9 Pyrin domain suggests a distinct mode of inflammasome assembly. FEBS Lett. 594, 2383–2395.

Fan, Z., Devlin, J.R., Hogg, S.J., Doyle, M.A., Harrison, P.F., Todorovski, I., Cluse, L.A., Knight, D.A., Sandow, J.J., Gregory, G., Fox, A., Beilharz, T.H., Kwiatkowski, N., Scott, N.E., Vidakovic, A.T., Kelly, G.P., Svejstrup, J.Q., Geyer, M., Gray, N.S., Vervoort, S.J., and Johnstone, R.W. (2020). CDK13 cooperates with CDK12 to control global RNA polymerase II processivity. Sci. Adv. 6:eaaz5041.

Friker, L.L., Scheiblich, H., Hochheiser, I.V., Brinkschulte, R., Riedel, D., Latz, E., Geyer, M., and Heneka, M.T. (2020). β-Amyloid clustering around ASC fibrils boosts its toxicity in microglia. Cell Rep. 30, 3743–3754.

2019
Torres-Fernández, L.A., Jux, B., Bille, M., Port, Y., Schneider, K., Geyer, M., Mayer, G., and Kolanus, W. (2019). The mRNA repressor TRIM71 cooperates with nonsense-mediated decay factors to destabilize the mRNA of CDKN1A/p21. Nucleic Acids Res. 47, 11861–11879.

Hoss, F., Mueller, J.L., Rojas Ringeling, F., Rodriguez-Alcazar, J.F., Brinkschulte, R., Seifert, G., Stahl, R., Broderick, L., Putnam, C.D., Kolodner, R.D., Canzar, S., Geyer, M., Hoffman, H.M., and Latz, E. (2019). Alternative splicing regulates stochastic NLRP3 activity. Nat. Commun. 10:3238.

Olson, C.M., Liang, Y., Leggett, A., Park, W.D., Li, L., Mills, C.E., Elsarrag, S.Z., Ficarro, S.B., Zhang, T., Düster, R., Geyer, M., Sim, T., Marto, J.A., Sorger, P.K., Westover, K.D., Lin, C.Y., Kwiatkowski, N., and Gray, N.S. (2019). Development of a selective CDK7 covalent inhibitor reveals predominant cell-cycle phenotype. Cell Chem. Biol. 26, 792–803.

Baluapuri, A., Hofstetter, J., Dudvarski Stankovic, N., Endres, T., Bhandare, P., Vos, S.M., Adhikari, B., Schwarz, J.D., Narain, A., Vogt, M., Wang, S.Y., Düster, R., Jung, L.A., Vanselow, J.T., Wiegering, A., Geyer, M., Maric, H.M., Gallant, P., Walz, S., Schlosser, A., Cramer, P., Eilers, M., and Wolf, E. (2019). MYC recruits SPT5 to RNA polymerase II to promote processive transcription elongation. Mol. Cell 74, 674–687.

Bugai, A., Quaresma, A.J.C., Friedel, C.C., Lenasi, T., Düster, R., Sibley, C.R., Fujinaga, K., Kukanja, P., Hennig, T., Blasius, M., Geyer, M., Ule, J., Dölken, L., and Barborič, M. (2019). P-TEFb activation by RBM7 shapes a pro-survival transcriptional response to genotoxic stress. Mol. Cell 74, 254–267.

Krajewska, M., Dries, R., Grassetti, A.V., Dust, S., Gao, Y., Huang, H., Sharma, B., Day, D.S., Kwiatkowski, N., Pomaville, M., Dodd, O., Chipumuro, E., Zhang, T., Greenleaf, A.L., Yuan, G.C., Gray, N.S., Young, R.A., Geyer, M., Gerber, S.A., and George, R.E. (2019). CDK12 loss in cancer cells affects DNA damage response genes through premature cleavage and polyadenylation. Nat. Commun. 10:1757.

Grueb, S.S., Muhs, S., Popp, Y., Schmitt, S., Geyer, M., Lin, Y.N., and Windhorst, S. (2019). The formin Drosophila homologue of Diaphanous2 (Diaph2) controls microtubule dynamics in colorectal cancer cells independent of its FH2-domain. Sci. Rep. 9:5352.

Woeste, M.A., Stern, S., Raju, D.N., Grahn, E., Dittmann, D., Gutbrod, K., Dörmann, P., Hansen, J.N., Schonauer, S., Marx, C.E., Hamzeh, H., Körschen, H.G., Aerts, J.M.F.G., Bōnigk, W., Endepols, H., Sandhoff, R., Geyer, M., Berger, T.K., Bradke, F., and Wachten, D. (2019). Species-specific differences in non-lysosomal glucosylceramidase GBA2 function underlie locomotor dysfunction arising from loss-of-function mutations. J. Biol. Chem. 294, 3853–3871.

Rohrmoser, M., Kluge, M., Yahia, Y., Gruber-Eber, A., Maqbool, M.A., Forné, I., Krebs, S., Blum, H., Greifenberg, A.K., Geyer, M., Descostes, N., Imhof, A., Andrau, J.C., Friedel, C.C., and Eick, D. (2019). MIR sequences recruit zinc finger protein ZNF768 to expressed genes. Nucleic Acids Res. 47, 700–715.

2018
Romano, M.T., Tafazzoli, A., Mattern, M., Sivalingam, S., Wolf, S., Rupp, A., Thiele, H., Altmüller, J., Nürnberg, P., Ellwanger, J., Gambon, R., Baumer, A., Kohlschmidt, N., Metze, D., Holdenrieder, S., Paus, R., Lütjohann, D., Frank, J., Geyer, M., Bertolini, M., Kokordelis, P., and Betz, R.C. (2018). Bi-allelic mutations in LSS, encoding lanosterol synthase, cause autosomal-recessive hypotrichosis simplex. Am. J. Hum. Genet. 103, 777–785.

Müller, J.A., Glöckle, A., Gawanbacht, A., Geyer, M., Münch, J., and Kirchhoff, F. (2018). Reduced susceptibility to VIRIP-based HIV-1 entry inhibitors has a high genetic barrier and severe fitness costs. J. Virol. 92, e00733-18.

Yu, C.F., Peng, W.M., Schlee, M., Barchet, W., Eis-Hübinger, A.M., Kolanus, W., Geyer, M., Schmitt, S., Steinhagen, F., Oldenburg, J., and Novak, N. (2018). SOCS1 and SOCS3 target IRF7 degradation to suppress TLR7-mediated type I IFN production of human plasmacytoid dendritic cells. J. Immunol. 200, 4024–4035.

Yu, C.H., Moecking, J., Geyer, M., and Masters, S.L. (2018). Mechanisms of NLRP1-mediated autoinflammatory disease in humans and mice. J. Mol. Biol. 430, 142–152.

2017
Venegas, C., Kumar, S., Franklin, B.S., Dierkes, T., Brinkschulte, R., Tejera, D., Vieira-Saecker, A., Schwartz, S., Santarelli, F., Kummer, M.P., Griep, A., Gelpi, E., Beilharz, M., Riedel, D., Golenbock, D.T., Geyer, M., Walter, J., Latz, E., and Heneka, M.T. (2017). Microglia-derived ASC specks cross-seed amyloid-β in Alzheimer’s disease. Nature 552, 355–361.